Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68877-1-Ig targets ADCK4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13188 Product name: Recombinant human ADCK4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 195-544 aa of BC013114 Sequence: LEEELGRDWQAKVASLEEVPFAAASIGQVHQGLLRDGTEVAVKIQYPGIAQSIQSDVQNLLAVLKMSAALPAGLFAEQSLQALQQELAWECDYRREAACAQNFRQLLANDPFFRVPAVVKELCTTRVLGMELAGGVPLDQCQGLSQDLRNQICFQLLTLCLRELFEFRFMQTDPNWANFLYDASSHQVTLLDFGASREFGTEFTDHYIEVVKAAADGDRDCVLQKSRDLKFLTGFETKAFSDAHVEAVMILGEPFATQGPYDFGSGETARRIQDLIPVLLRHRLCPPPEETYALHRKLAGAFLACAHLRAHIACRDLFQDTYHRYWASRQPDAATAGSLPTKGDSWVDPS 相同性解析による交差性が予測される生物種 |
| Full Name | aarF domain containing kinase 4 |
| Calculated molecular weight | 544 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC013114 |
| Gene Symbol | ADCK4 |
| Gene ID (NCBI) | 79934 |
| RRID | AB_3670444 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96D53 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADCK4 (AarF domain-containing protein kinase 4), also known as COQ8B (Coenzyme Q protein 8B), is an atypical kinase involved in the biosynthesis of coenzyme Q, which is also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PMID: 24270420). Its substrate specificity is unclear: does not show any protein kinase activity. It probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway (PMID: 24270420).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADCK4 antibody 68877-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
