Validation Data Gallery
Tested Applications
| Positive WB detected in | HaCaT cells, A431 cells, SW 1990 cells, HT-29 cells, MDA-MB-468 cells, HeLa cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68868-1-Ig targets MYO18A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6185 Product name: Recombinant human MYO18A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1601-2002 aa of BC039612 Sequence: TLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA 相同性解析による交差性が予測される生物種 |
| Full Name | myosin XVIIIA |
| Calculated molecular weight | 233 kDa |
| Observed molecular weight | 230 kDa, 190 kDa |
| GenBank accession number | BC039612 |
| Gene Symbol | MYO18A |
| Gene ID (NCBI) | 399687 |
| RRID | AB_3670442 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92614 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYO18A antibody 68868-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |