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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AZI2 Monoclonal antibody (68326-1-Ig)

    WB analysis using 68326-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68326-1-Ig (AZI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AZI2 Monoclonal antibody (68326-1-Ig)

    IF Staining of HeLa using 68326-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AZI2 antibody (68326-1-Ig, Clone: 2F1G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

AZI2 Monoclonal antibody

AZI2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68326-1-Ig
Clone No.2F1G5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AZI2/NAP1, 2F1G5, 5 azacytidine induced 2, 5-azacytidine-induced protein 2, Nak associated protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, MDA-MB-231 cells, SK-BR-3 cells, LNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68326-1-Ig targets AZI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6755

Product name: Recombinant human AZI2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-264 aa of BC001139

Sequence: MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKRLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVCIDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKGKSLFTGNH

相同性解析による交差性が予測される生物種
Full Name 5-azacytidine induced 2
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC001139
Gene Symbol AZI2
Gene ID (NCBI) 64343
RRIDAB_2935398
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9H6S1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. NAP1 is a kind of adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. (PMID: 21931631)

Protocols

Product Specific Protocols
WB protocol for AZI2 antibody 68326-1-IgDownload protocol
IF protocol for AZI2 antibody 68326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols