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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 18 Monoclonal antibody (68220-1-Ig)

    WB analysis using 68220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using Syntaxin 18 Monoclonal antibody (68220-1-Ig)

    WB analysis using 68220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using Syntaxin 18 Monoclonal antibody (68220-1-Ig)

    WB analysis of pig brain using 68220-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.

Syntaxin 18 Monoclonal antibody

Syntaxin 18 Monoclonal Antibody for WB, ELISA
Cat No. 68220-1-Ig
Clone No.2A2C9

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

DKFZp686O15149, STX18, syntaxin 18, Ufe1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, rat brain tissue, pig brain tissue, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68220-1-Ig targets Syntaxin 18 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8947

Product name: Recombinant human Syntaxin 18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-317 aa of BC014613

Sequence: MAVDITLLFRASVKTVKTRNKALGVAVGGGVDGSRDELFRRSPRPKGDFSSRAREVISHIGKLRDFLLEHRKDYINAYSHTMSEYGRMTDTERDQIDQDAQIFMRTCSEAIQQLRTEAHKEIHSQQVKEHRTAVLDFIEDYLKRVCKLYSEQRAIRVKRVVDKKRLSKLEPEPNTKTRESTSSEKVSQSPSKDSEENPATEERPEKILAETQPELGTWGDGKGEDELSPEEIQMFEQENQRLIGEMNSLFDEVRQIEGRVVEISRLQEIFTEKVLQQEAEIDSIHQLVVGATENIKEGNEDIREAIKNNAGFRVWIL

相同性解析による交差性が予測される生物種
Full Name syntaxin 18
Calculated molecular weight 335 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC014613
Gene Symbol Syntaxin 18
Gene ID (NCBI) 53407
RRIDAB_2935308
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9P2W9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin 18 is a novel member of the syntaxin family and it is involved in ER-mediated phagocytosis by regulating the specific and direct fusion of the ER and plasma or phagosomal membranes( PMID: 16790498).

Protocols

Product Specific Protocols
WB protocol for Syntaxin 18 antibody 68220-1-IgDownload protocol
FC protocol for Syntaxin 18 antibody 68220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols