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Validation Data Gallery

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  • Western Blot (WB) analysis of hTERT-RPE1 cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    WB analysis of hTERT-RPE1 using 68212-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    WB analysis of U-87 MG using 68212-1-Ig

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    IF Staining of HeLa using 68212-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    IF Staining of HeLa using 68212-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

NOTCH2/NOTCH2NL Monoclonal antibody

NOTCH2/NOTCH2NL Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68212-1-Ig
Clone No.2D5E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhTERT-RPE1 cells, U-87 MG cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68212-1-Ig targets NOTCH2/NOTCH2NL in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1679

Product name: Recombinant human NOTCH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-254 aa of BC019835

Sequence: PAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGICLNLPGSYQCQCLQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPETVRRGTELWERDREVWNGKEHDEN

相同性解析による交差性が予測される生物種
Full Name Notch homolog 2 (Drosophila) N-terminal like
Calculated molecular weight 265 kDa
Observed molecular weight250-265 kDa
GenBank accession numberBC019835
Gene Symbol NOTCH2NL
Gene ID (NCBI) 388677
RRIDAB_2935300
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP0DPK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Notch 2 is a member of the notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch2, like other Norch genes, functions in development of various tissues. This antibody can recognize NOTCH2 and NOTCH2NL. The antibody detects the full-length NOTCH2 protein about 250-265 kDa.

Protocols

Product Specific Protocols
IF protocol for NOTCH2/NOTCH2NL antibody 68212-1-IgDownload protocol
WB protocol for NOTCH2/NOTCH2NL antibody 68212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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