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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL12 Monoclonal antibody (68110-1-Ig)

    WB analysis using 68110-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68110-1-Ig (RPL12 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using RPL12 Monoclonal antibody (68110-1-Ig)

    WB analysis of HeLa using 68110-1-Ig

    WB result of RPL12 antibody (68110-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL12 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of human endometrial cancer using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of mouse brain using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of mouse stomach using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of rat brain using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of rat stomach using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of human placenta using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

RPL12 Monoclonal antibody

RPL12 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68110-1-Ig
Clone No.2C9B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2C9B3, 60S ribosomal protein L12, Large ribosomal subunit protein uL11, ribosomal protein L12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human endometrial cancer tissue, human placenta tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68110-1-Ig targets RPL12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6580

Product name: Recombinant human RPL12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-165 aa of BC050644

Sequence: MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAVECPAS

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L12
Calculated molecular weight 18 kDa
Observed molecular weight21 kDa
GenBank accession numberBC050644
Gene Symbol RPL12
Gene ID (NCBI) 6136
RRIDAB_2923639
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP30050
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for RPL12 antibody 68110-1-IgDownload protocol
WB protocol for RPL12 antibody 68110-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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