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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMG6 Monoclonal antibody (68109-1-Ig)

    WB analysis using 68109-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of RAW 264.7 cells using SMG6 Monoclonal antibody (68109-1-Ig)

    WB analysis of RAW 264.7 using 68109-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SMG6 Monoclonal antibody

SMG6 Monoclonal Antibody for WB, ELISA
Cat No. 68109-1-Ig
Clone No.1D2G2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

C17orf31, EST1 like protein A, EST1A, Ever shorter telomeres 1A, hSmg5/7a, KIAA0732, SMG 6, Smg 6 homolog, SMG6, Telomerase subunit EST1A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, RAW 264.7 cells, MCF-7 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68109-1-Ig targets SMG6 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30182

Product name: Recombinant human SMG6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1282-1419 aa of NM_017575

Sequence: ELDGLAKGQETDHRAGGYARVVQEKARKSIEFLEQRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDFMPASKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

相同性解析による交差性が予測される生物種
Full Name Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Calculated molecular weight 160 kDa
Observed molecular weight160 kDa
GenBank accession numberNM_017575
Gene Symbol SMG6
Gene ID (NCBI) 23293
RRIDAB_2923638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ86US8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMG6, also known as C17orf31, EST1A, and hEST1A, is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PMID: 19179534). It promotes in vitro the ability of TERT to elongate telomeres (PMID: 12676087, 12699629). SMG6 also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD (PMID: 19060897, 20930030). Alternate splicing results in multiple transcript variants (PMID: 12699629, 14636577).

Protocols

Product Specific Protocols
WB protocol for SMG6 antibody 68109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols