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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN9 Monoclonal antibody (67931-1-Ig)

    WB analysis using 67931-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PTPN9 Monoclonal antibody (67931-1-Ig)

    WB analysis of A549 using 67931-1-Ig

    WB result of PTPN9 antibody (67931-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN9 transfected A549 cells.

  • Western Blot (WB) analysis of pig pancreas tissue using PTPN9 Monoclonal antibody (67931-1-Ig)

    WB analysis of pig pancreas using 67931-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTPN9 Monoclonal antibody (67931-1-Ig)

    WB analysis using 67931-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PTPN9 Monoclonal antibody (67931-1-Ig)

    IF Staining of U2OS using 67931-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PTPN9 antibody (67931-1-Ig, Clone: 1D6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

PTPN9 Monoclonal antibody

PTPN9 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67931-1-Ig
Clone No.1D6E4

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MEG2, PTPase MEG2, PTPMEG2, PTPN9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, pig pancreas tissue, A549 cells, Hela cells, RAW 264.7 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67931-1-Ig targets PTPN9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30615

Product name: Recombinant human PTPN9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-593 aa of BC010863

Sequence: FQFLPQVNGHPDPFDEIILFSLPPALDWDSVHVPGPHAMTIQELVDYVNARQKQGIYEEYEDIRRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGHTQTDYINASFMDGYKQKNAYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKCGQYWPLEKDSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYGVPSSAASLIDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICLAQLEELGTLNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVESQ

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 9
Calculated molecular weight 593 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC010863
Gene Symbol PTPN9
Gene ID (NCBI) 5780
RRIDAB_2918683
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP43378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177).

Protocols

Product Specific Protocols
WB protocol for PTPN9 antibody 67931-1-IgDownload protocol
IF protocol for PTPN9 antibody 67931-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols