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ROBO1 Monoclonal antibody

ROBO1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4H5

Cat no : 67922-1-Ig

Synonyms

Deleted in U twenty twenty, DUTT1, FLJ21882, H Robo 1, ROBO1, Roundabout homolog 1, SAX3

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using ROBO1 Monoclonal antibody (67922-1-Ig)

    WB analysis of Y79 using 67922-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using ROBO1 Monoclonal antibody (67922-1-Ig)

    WB analysis of U-87 MG using 67922-1-Ig

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ROBO1 Monoclonal antibody (67922-1-Ig)

    WB analysis of HeLa using 67922-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ROBO1 Monoclonal antibody (67922-1-Ig)

    WB analysis of HEK-293 using 67922-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ROBO1 Monoclonal antibody (67922-1-Ig)

    WB analysis of HepG2 using 67922-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ROBO1 Monoclonal antibody (67922-1-Ig)

    IHC staining of mouse cerebellum using 67922-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ROBO1 Monoclonal antibody (67922-1-Ig)

    IHC staining of mouse cerebellum using 67922-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ROBO1 Monoclonal antibody (67922-1-Ig)

    IHC staining of mouse cerebellum using 67922-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ROBO1 Monoclonal antibody (67922-1-Ig)

    IHC staining of mouse cerebellum using 67922-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, HeLa cells, HepG2 cells, U-87 MG cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67922-1-Ig targets ROBO1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ROBO1 fusion protein Ag18074
Full Name roundabout, axon guidance receptor, homolog 1 (Drosophila)
Calculated Molecular Weight 1651 aa, 181 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC112336
Gene Symbol ROBO1
Gene ID (NCBI) 6091
RRIDAB_2918674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.

Protocols

Product Specific Protocols
WB protocol for ROBO1 antibody 67922-1-IgDownload protocol
IHC protocol for ROBO1 antibody 67922-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols