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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMO4 Monoclonal antibody (67881-1-Ig)

    WB analysis using 67881-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HuH-7 cells using FMO4 Monoclonal antibody (67881-1-Ig)

    WB analysis of HuH-7 using 67881-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using FMO4 Monoclonal antibody (67881-1-Ig)

    IF Staining of mouse liver using 67881-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using FMO4 Monoclonal antibody (67881-1-Ig)

    IF Staining of mouse liver using 67881-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HuH-7 cells using FMO4 Monoclonal antibody (67881-1-Ig)

    FC experiment of HuH-7 using 67881-1-Ig

    1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

FMO4 Monoclonal antibody

FMO4 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 67881-1-Ig
Clone No.2D12E10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

2D12E10, Dimethylaniline monooxygenase [N-oxide-forming] 4, EC:1.14.13.8, FMO2, Hepatic flavin-containing monooxygenase 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrabbit liver tissue, HuH-7 cells, pig liver tissue, rat liver tissue, mouse liver tissue
Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67881-1-Ig targets FMO4 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7935

Product name: Recombinant human FMO4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 24-354 aa of BC002780

Sequence: EDLEPTCFERSDDIGGLWKFTESSKDGMTRVYKSLVTNVCKEMSCYSDFPFHEDYPNFMNHEKFWDYLQEFAEHFDLLKYIQFKTTVCSITKRPDFSETGQWDVVTETEGKQNRAVFDAVMVCTGHFLNPHLPLEAFPGIHKFKGQILHSQEYKIPEGFQGKRVLVIGLGNTGGDIAVELSRTAAQVLLSTRTGTWVLGRSSDWGYPYNMMVTRRCCSFIAQVLPSRFLNWIQERKLNKRFNHEDYGLSITKGKKAKFIVNDELPNCILCGAITMKTSVIEFTETSAVFEDGTVEENIDVVIFTTGYTFSFPFFEEPLKSLCTKKIFLYKQ

相同性解析による交差性が予測される生物種
Full Name flavin containing monooxygenase 4
Calculated molecular weight 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC002780
Gene Symbol FMO4
Gene ID (NCBI) 2329
RRIDAB_2918638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP31512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for FMO4 antibody 67881-1-IgDownload protocol
IF protocol for FMO4 antibody 67881-1-IgDownload protocol
WB protocol for FMO4 antibody 67881-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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