WWOX Monoclonal antibody

WWOX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8F4

Cat no : 67725-1-Ig

Synonyms

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, pig liver tissue, rat liver tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67725-1-Ig targets WWOX in WB, IHC, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Reactivity Human, rat, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen WWOX fusion protein Ag7969
Full Name WW domain containing oxidoreductase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC003184
Gene Symbol WWOX
Gene ID (NCBI) 51741
RRIDAB_2882911
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for WWOX antibody 67725-1-IgDownload protocol
IHC protocol for WWOX antibody 67725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols