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PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G8C1

Cat no : 67562-1-Ig

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PAK6 Monoclonal antibody (67562-1-Ig)

    WB analysis of K-562 using 67562-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PAK6 Monoclonal antibody (67562-1-Ig)

    WB analysis of HEK-293 using 67562-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PAK6 Monoclonal antibody (67562-1-Ig)

    IF Staining of HEK-293 using 67562-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PAK6 antibody (67562-1-Ig, Clone: 3G8C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67562-1-Ig targets PAK6 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAK6 fusion protein Ag27692
Full Name p21 protein (Cdc42/Rac)-activated kinase 6
Calculated Molecular Weight 681 aa, 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC035596
Gene Symbol PAK6
Gene ID (NCBI) 56924
RRIDAB_2882776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).

Protocols

Product Specific Protocols
WB protocol for PAK6 antibody 67562-1-IgDownload protocol
IF protocol for PAK6 antibody 67562-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols