Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, NIH/3T3 cells, HEK-293 cells, LNCaP cells, Hela cells, HepG cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67540-1-Ig targets TCP1 eta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9502 Product name: Recombinant human CCT7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 240-543 aa of BC019296 Sequence: LNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVDAPTAAGRGRGRGRPH 相同性解析による交差性が予測される生物種 |
| Full Name | chaperonin containing TCP1, subunit 7 (eta) |
| Calculated molecular weight | 543 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC019296 |
| Gene Symbol | CCT7 |
| Gene ID (NCBI) | 10574 |
| RRID | AB_2882759 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99832 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TCP1 eta antibody 67540-1-Ig | Download protocol |
| IHC protocol for TCP1 eta antibody 67540-1-Ig | Download protocol |
| WB protocol for TCP1 eta antibody 67540-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
