Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, LNCaP cells, IMR-32 cells, Daudi cells, NK-92 cells, HepG2 cells, Raji cells, rabbit brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
67534-1-Ig targets RALYL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11676 Product name: Recombinant human RALYL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-291 aa of BC031090 Sequence: MTGKTQTSNVTNKNDPKSINSRVFIGNLNTAIVKKVDIEAIFSKYGKIVGCSVHKGYAFVQYMSERHARAAVAGENARVIAGQPLDINMAGEPKPYRPKPGNKRPLSALYRLESKEPFLSVGGYVFDYDYYRDDFYNRLFDYHGRVPPPPRAVIPLKRPRVAVTTTRRGKGVFSMKGGSRSTASGSTGSKLKSDELQTIKKELTQIKTKIDSLLGRLEKIEKQQKAEAEAQKKQLEESLVLIQEECVSEIADHSTEEPAEGGPDADGEEMTDGIEEDFDEDGGHELFLQIK 相同性解析による交差性が予測される生物種 |
| Full Name | RALY RNA binding protein-like |
| Calculated molecular weight | 291 aa, 32 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC031090 |
| Gene Symbol | RALYL |
| Gene ID (NCBI) | 138046 |
| RRID | AB_2882753 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86SE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RALYL antibody 67534-1-Ig | Download protocol |
| WB protocol for RALYL antibody 67534-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
