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Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using AMT Monoclonal antibody (67532-1-Ig)

    WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using AMT Monoclonal antibody (67532-1-Ig)

    WB analysis of rat liver using 67532-1-Ig (same clone as 67532-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using AMT Monoclonal antibody (67532-1-Ig)

    WB analysis of mouse liver using 67532-1-Ig (same clone as 67532-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AMT Monoclonal antibody (67532-1-Ig)

    IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AMT Monoclonal antibody (67532-1-Ig)

    IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AMT Monoclonal antibody (67532-1-Ig)

    IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50403-2

    Cytometric bead array standard curve of MP50403-2

    Cytometric bead array standard curve of MP50403-2, AMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67532-3-PBS. Detection antibody: 67532-1-PBS. Standard:Ag26644. Range: 3.125-100 ng/mL.

  • Affinity and Kinetic Characterization of 67532-1-Ig

    Affinity and Kinetic Characterization of 67532-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 67532-1-Ig against Human AMT was performed. The affinity constant is 8.85 nM.

AMT Monoclonal antibody, PBS Only (Detector)

AMT Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67532-1-PBS
Clone No.3E7D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

GCST, 3E7D4, aminomethyltransferase, EC:2.1.2.10, GCVT

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67532-1-PBS targets AMT as part of a matched antibody pair:

MP50403-2: 67532-3-PBS capture and 67532-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26644

Product name: Recombinant human AMT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 147-321 aa of BC007546

Sequence: EKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLM

相同性解析による交差性が予測される生物種
Full Name aminomethyltransferase
Calculated molecular weight 44 kDa
Observed molecular weight37-44 kDa
GenBank accession numberBC007546
Gene Symbol AMT
Gene ID (NCBI) 275
RRIDAB_2882751
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP48728
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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