Various lysates were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 67480-1-Ig incubated at 1:10000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HepG2 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Jurkat cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

pig brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

rat brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human SOD1 (67480-1-Ig, Clone:2F10G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.