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ZNF746 Monoclonal antibody

ZNF746 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A12C5

Cat no : 67463-1-Ig

Synonyms

FLJ31413, PARIS, zinc finger protein 746, ZNF746

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ZNF746 Monoclonal antibody (67463-1-Ig)

    WB analysis of HEK-293 using 67463-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using ZNF746 Monoclonal antibody (67463-1-Ig)

    WB analysis of LNCaP using 67463-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67463-1-Ig targets ZNF746 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZNF746 fusion protein Ag21582
Full Name zinc finger protein 746
Calculated Molecular Weight 644 aa, 69 kDa
Observed Molecular Weight85-90 kDa
GenBank Accession NumberBC068505
Gene Symbol ZNF746
Gene ID (NCBI) 155061
RRIDAB_2918491
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN: its accumulation due to the absence of PRKN, followed by up-regulation of PPARGC1A, could lead to the selective loss of dopamine neurons in the substantia nigra(PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

Protocols

Product Specific Protocols
WB protocol for ZNF746 antibody 67463-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols