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TH1L Monoclonal antibody

TH1L Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10G2

Cat no : 67380-1-Ig

Synonyms

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis using 67380-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of RAW 264.7 cells using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis of RAW 264.7 using 67380-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis of Jurkat using 67380-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis of Jurkat using 67380-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis of K-562 using 67380-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis of HSC-T6 using 67380-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TH1L Monoclonal antibody (67380-1-Ig)

    WB analysis of NIH/3T3 using 67380-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TH1L Monoclonal antibody (67380-1-Ig)

    IF Staining of HeLa using 67380-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67380-1-Ig (TH1L antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TH1L Monoclonal antibody (67380-1-Ig)

    IF Staining of HeLa using 67380-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TH1L antibody (67380-1-Ig, Clone: 1A10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TH1L Monoclonal antibody (67380-1-Ig)

    IF Staining of HeLa using 67380-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TH1L antibody (67380-1-Ig, Clone: 1A10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using TH1L Monoclonal antibody (67380-1-Ig)

    FC experiment of HeLa using 67380-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TH1L (67380-1-Ig, Clone:1A10G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, MCF-7 cells, HeLa cells, HepG2 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67380-1-Ig targets TH1L in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TH1L fusion protein Ag24894
Full Name TH1-like (Drosophila)
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC014952
Gene Symbol TH1L
Gene ID (NCBI) 51497
RRIDAB_2882627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TH1L antibody 67380-1-IgDownload protocol
IF protocol for TH1L antibody 67380-1-IgDownload protocol
FC protocol for TH1L antibody 67380-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols