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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of human placenta using 67344-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of NCCIT using 67344-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of JAR using 67344-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of HepG2 using 67344-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of JAR using 67344-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human prostate hyperplasia using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human prostate hyperplasia using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 40x lens)..

  • Immunofluorescence (IF) / fluorescent staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FUT3 Monoclonal antibody

FUT3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67344-1-Ig
Clone No.2A6G7
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, NCCIT cells, JAR cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate hyperplasia tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67344-1-Ig targets FUT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12581

Product name: Recombinant human FUT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-361 aa of BC108675

Sequence: MVPGTADCHITADRKVYPQADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWALDFCKACWKLQQESRYQTVRSIAAWFT

相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC108675
Gene Symbol FUT3
Gene ID (NCBI) 2525
RRIDAB_2882602
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP21217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for FUT3 antibody 67344-1-IgDownload protocol
IHC protocol for FUT3 antibody 67344-1-IgDownload protocol
WB protocol for FUT3 antibody 67344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

NPJ Precis Oncol

FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis.

Authors - Kaiwen Qin
View Article
humanWB,IHC

Eur J Med Res

FUT3-B3GNT3 interaction promotes pancreatic cancer progression and chemoresistance via NF-κB signaling mediated autophagy.

Authors - Lei Jin
  • KD Validated
View Article
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