WWP2 Monoclonal antibody

WWP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9B4

Cat no : 67274-1-Ig

Synonyms

AIP2, WW domain containing protein 2, WWP2, WWp2 like



Tested Applications

Positive WB detected inHEK-293 cells, LNCap cells, MCF-7 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67274-1-Ig targets WWP2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WWP2 fusion protein Ag2835
Full Name WW domain containing E3 ubiquitin protein ligase 2
Calculated Molecular Weight 870 aa, 99 kDa
Observed Molecular Weight 100-115 kDa
GenBank Accession NumberBC013645
Gene Symbol WWP2
Gene ID (NCBI) 11060
RRIDAB_2882543
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WWP2 (WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes (PMID:21532586). WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells (PMID:15047715).

Protocols

Product Specific Protocols
WB protocol for WWP2 antibody 67274-1-IgDownload protocol
IHC protocol for WWP2 antibody 67274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer

Authors - Liang Xu