Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, pig brain tissue, Y79 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67181-1-Ig targets GAS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.
| Tested Reactivity | Human, Mouse, Pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12259 Product name: Recombinant human GAS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-345 aa of BC074908 Sequence: LQLLGSAPRGSGLAHGRRLICWQALLQCQGEPECSYAYNQYAEACAPVLAQHGGGDAPGAAAAAFPASAASFSSRWRCPSHCISALIQLNHTRRGPALEDCDCAQDENCKSTKRAIEPCLPRTSGGGAGGPGAGGVMGCTEARRRCDRDSRCNLALSRYLTYCGKVFNGLRCTDECRTVIEDMLAMPKAALLNDCVCDGLERPICESVKENMARLCFGAELGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGGEQPLDDDDGVPHPPRPGSGAAASGGRGDLPYGPGRRSSGGGGRLAPRGAWTPLASILLLLLGPLF 相同性解析による交差性が予測される生物種 |
| Full Name | growth arrest-specific 1 |
| Calculated molecular weight | 345 aa, 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC074908 |
| Gene Symbol | GAS1 |
| Gene ID (NCBI) | 2619 |
| RRID | AB_2882477 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54826 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAS1 antibody 67181-1-Ig | Download protocol |
| WB protocol for GAS1 antibody 67181-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
