Validation Data Gallery

Filter: AllWBIPIHCIF/ICCCytometric bead array

WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)
WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)
IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50161-1
Cytometric bead array standard curve of MP50161-1, DHX9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67153-2-PBS. Detection antibody: 67153-1-PBS. Standard:Ag12104. Range: 1.563-100 ng/mL.

Featured Product
KD/KO Validated

DHX9 Monoclonal antibody, PBS Only (Detector)

DHX9 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Cat No. 67153-1-PBS

Clone No.1B12C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Synonyms

1B12C10, ATP-dependent RNA helicase A, DDX9, DExH-box helicase 9, EC:3.6.4.13

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67153-1-PBS targets DHX9 as part of a matched antibody pair:

MP50161-1: 67153-2-PBS capture and 67153-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag12104 Product name: Recombinant human DHX9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC014246 Sequence: MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPP 相同性解析による交差性が予測される生物種
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight	1270 aa, 141 kDa
Observed molecular weight	140 kDa
GenBank accession number	BC014246
Gene Symbol	DHX9
Gene ID (NCBI)	1660
RRID	AB_2882450
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q08211
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

WB analysis of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

WB analysis of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)