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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of HeLa using 67113-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of MCF-7 using 67113-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of Jurkat using 67113-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of HSC-T6 using 67113-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of NIH/3T3 using 67113-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of HepG2 using 67113-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of A431 using 67113-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

PKC Delta Monoclonal antibody

PKC Delta Monoclonal Antibody for WB, ELISA
Cat No. 67113-1-Ig
Clone No.2B3E3
Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

MAY1, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, A431 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67113-1-Ig targets PKC Delta in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5631

Product name: Recombinant human PRKCD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 326-676 aa of BC043350

Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

相同性解析による交差性が予測される生物種
Full Name protein kinase C, delta
Calculated molecular weight 78 kDa
Observed molecular weight 78-80 kDa
GenBank accession numberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
RRIDAB_2882417
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Delta antibody 67113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Int Immunopharmacol

Osthole alleviates neuropathic pain by suppressing astrocytes activation and associated inflammatory responses via the PKCδ/TRPV4 signaling pathway

Authors - Xiaoqian Xu
View Article
mouseWB

Cell Death Differ

The PM20D1-NADA pathway protects against Parkinson's disease

Authors - Yunying Yang
View Article
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