Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Caco-2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HepG2 cells, K-562 cells, PC-12 cells, Neuro-2a cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
| RIP | See 1 publications below |
Product Information
67109-1-Ig targets CNBP in WB, IF, IP, RIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7114 Product name: Recombinant human CNBP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-170 aa of BC000288 Sequence: MSSNECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA 相同性解析による交差性が予測される生物種 |
| Full Name | CCHC-type zinc finger, nucleic acid binding protein |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 19-25 kDa |
| GenBank accession number | BC000288 |
| Gene Symbol | CNBP |
| Gene ID (NCBI) | 7555 |
| RRID | AB_2882413 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CNBP antibody 67109-1-Ig | Download protocol |
| WB protocol for CNBP antibody 67109-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Microbiol The SARS-CoV-2 RNA-protein interactome in infected human cells.
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PLoS One CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA | ||
J Mol Cell Cardiol Cellular nucleic acid binding protein facilitates cardiac repair after myocardial infarction by activating β-catenin signaling | ||
PLoS One circTADA2A inhibited SLC38A1 expression and suppresses melanoma progression through the prevention of CNBP trans-activation | ||
bioRxiv A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions
| ||
Cell Rep A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions
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