Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue, human heart tissue, pig heart tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 17 publications below |
| IF | See 3 publications below |
Product Information
67082-1-Ig targets Myogenin in WB, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.
| Tested Reactivity | Human, Rat, Pig |
| Cited Reactivity | human, mouse, rabbit, bovine, goat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6414 Product name: Recombinant human Myogenin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC053899 Sequence: MELYETSPYFYQEPRFYDGENYLPVHLQGFEPPGYERTELTLSPEAPGPLEDKGLGTPEHCPGQCLPWACKVCKRKSVSVDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPGVPSECSSHSASCSPEWGSALEFSANPGDHLLTADPTDAHNLHSLTSIVDSITVEDVSVAFPDETMPN 相同性解析による交差性が予測される生物種 |
| Full Name | myogenin (myogenic factor 4) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC053899 |
| Gene Symbol | Myogenin |
| Gene ID (NCBI) | 4656 |
| RRID | AB_2882390 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P15173 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Myogenin antibody 67082-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. | ||
Cell Mol Life Sci Ythdf2 facilitates precursor miR-378/miR-378-5p maturation to support myogenic differentiation | ||
Mol Ther Nucleic Acids Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. | ||
Int J Mol Sci G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle. | ||
Front Cell Dev Biol Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia. | ||
FASEB J Circular RNA circUSP13 sponges miR-29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1. |
