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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of MCF-7 using 66990-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of Jurkat using 66990-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of RAW 264.7 using 66990-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of HSC-T6 using 66990-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of NIH/3T3 using 66990-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of HeLa using 66990-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TP73 Monoclonal antibody (66990-1-Ig)

    WB analysis of HEK-293 using 66990-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TP73 Monoclonal antibody

TP73 Monoclonal Antibody for WB, ELISA
Cat No. 66990-1-Ig
Clone No.1D12A12
Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, RIP, ELISA

Synonyms

1D12A12, P73, tumor protein p73

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66990-1-Ig targets TP73 in WB, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26793

Product name: Recombinant human TP73 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-100 aa of NM_001126240

Sequence: MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYA

相同性解析による交差性が予測される生物種
Full Name tumor protein p73
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_001126240
Gene Symbol TP73
Gene ID (NCBI) 7161
RRIDAB_2882307
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO15350
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TP73 antibody 66990-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanRIP

Cell Rep

A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression

Authors - Ling Zhang
View Article
humanWB

Biochem Pharmacol

Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.

Authors - Xiaojiang Liu
View Article
humanWB

Carcinogenesis

Construction and identification of lncRNA/circRNA coregulated ceRNA networks in gemcitabine-resistant bladder carcinoma

Authors - Jingjing Pan
View Article
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