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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of HSC-T6 using 66989-1-Ig (same clone as 66989-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of NIH/3T3 using 66989-1-Ig (same clone as 66989-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of mouse liver using 66989-1-Ig (same clone as 66989-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using hIST1 Monoclonal antibody (66989-1-Ig)

    IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51578-1

    Cytometric bead array sample test of MP51578-1

    Cytometric bead array sample test of MP51578-1, hIST1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66989-1-PBS. Detection antibody: 66989-2-PBS.

  • Cytometric bead array standard curve of MP51578-1

    Cytometric bead array standard curve of MP51578-1

    Cytometric bead array standard curve of MP51578-1, hIST1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66989-1-PBS. Detection antibody: 66989-2-PBS. Standard:Ag28364. Range: 3.125-400 ng/mL.

hIST1 Monoclonal antibody, PBS Only (Capture)

hIST1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66989-1-PBS
Clone No.1E12A7

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

IST1, KIAA0174, 1E12A7, Charged multivesicular body protein 8, CHMP8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66989-1-PBS targets hIST1 as part of a matched antibody pair:

MP51578-1: 66989-1-PBS capture and 66989-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28364

Product name: Recombinant human hIST1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-360 aa of BC000430

Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS

相同性解析による交差性が予測される生物種
Full Name KIAA0174
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000430
Gene Symbol hIST1
Gene ID (NCBI) 9798
RRIDAB_2882306
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP53990
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

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