Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 50 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
66983-1-Ig targets TRPV1 in WB, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18683 Product name: Recombinant human TRPV1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-203 aa of BC132820 Sequence: MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQ 相同性解析による交差性が予測される生物種 |
| Full Name | transient receptor potential cation channel, subfamily V, member 1 |
| Calculated molecular weight | 839 aa, 95 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC132820 |
| Gene Symbol | TRPV1 |
| Gene ID (NCBI) | 7442 |
| RRID | AB_2882302 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NER1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPV1 antibody 66983-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Manganese oxide nanozyme-loaded CO-releasing halloysite nanotubes for remodeling colonic immune, microbiota, and intestinal barrier homeostasis with concurrent pain relief in ulcerative colitis. | ||
Nat Commun Nanoparticle-mediated TRPV1 channel blockade amplifies cancer thermo-immunotherapy via heat shock factor 1 modulation | ||
Adv Sci (Weinh) Near Infrared Responsive Gold Nanorods Attenuate Osteoarthritis Progression by Targeting TRPV1 | ||
Research (Wash D C) Bifunctional TRPV1 Targeted Magnetothermal Switch to Attenuate Osteoarthritis Progression | ||
J Control Release Ultrasound-responsive piezoelectric analgesic microspheres alleviate osteoarthritis pain. | ||
Cell Death Dis TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca2+/CaMKII/Nrf2 signaling pathway. |