Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HCT 116 cells, HSC-T6 cells, Raji cells, HT-29 cells |
| Positive IHC detected in | mouse liver tissue, human kidney tissue, rat liver tissue, mouse kidney tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66706-1-Ig targets MTAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17954 Product name: Recombinant human MTAP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-154 aa of BC018625 Sequence: MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDSHVRRACFPFTFHHDCFQRPPQKPSRSHCVSCATCRTMS 相同性解析による交差性が予測される生物種 |
| Full Name | methylthioadenosine phosphorylase |
| Calculated molecular weight | 283 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC018625 |
| Gene Symbol | MTAP |
| Gene ID (NCBI) | 4507 |
| RRID | AB_2882058 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTAP antibody 66706-1-Ig | Download protocol |
| IHC protocol for MTAP antibody 66706-1-Ig | Download protocol |
| WB protocol for MTAP antibody 66706-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
