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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP62 Monoclonal antibody (66573-1-Ig)

    WB analysis using 66573-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using NUP62 Monoclonal antibody (66573-1-Ig)

    WB analysis of RAW 264.7 using 66573-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUP62 Monoclonal antibody (66573-1-Ig)

    IHC staining of human prostate cancer using 66573-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUP62 Monoclonal antibody (66573-1-Ig)

    IHC staining of human prostate cancer using 66573-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NUP62 Monoclonal antibody

NUP62 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66573-1-Ig
Clone No.2G12B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN, 62 kDa nucleoporin

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, Raji cells, DU 145 cells, PC-3 cells, HepG2 cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66573-1-Ig targets NUP62 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4912

Product name: Recombinant human NUP62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 173-522 aa of BC014842

Sequence: IGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATTTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD

相同性解析による交差性が予測される生物種
Full Name nucleoporin 62kDa
Calculated molecular weight 522 aa, 53 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC014842
Gene Symbol NUP62
Gene ID (NCBI) 23636
RRIDAB_2881933
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP37198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Protocols

Product Specific Protocols
IHC protocol for NUP62 antibody 66573-1-IgDownload protocol
WB protocol for NUP62 antibody 66573-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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