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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL10 Monoclonal antibody (66556-1-Ig)

    WB analysis using 66556-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of Raji cells using BCL10 Monoclonal antibody (66556-1-Ig)

    WB analysis of Raji using 66556-1-Ig

    Raji, K-562, and HL-60 cells were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human tonsillitis using 66556-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human tonsillitis using 66556-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human lymphoma using 66556-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human lymphoma using 66556-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCL10 Monoclonal antibody (66556-1-Ig)

    IF Staining of HepG2 using 66556-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (66556-1-Ig, Clone: 2E8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Jurkat cells using BCL10 Monoclonal antibody (66556-1-Ig)

    FC experiment of Jurkat using 66556-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL10 Monoclonal antibody (66556-1-Ig, Clone:2E8G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

BCL10 Monoclonal antibody

BCL10 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66556-1-Ig
Clone No.2E8G5
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

2E8G5, B-cell CLL/lymphoma 10, B-cell lymphoma/leukemia 10, Bcl 10, Bcl-10

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, HCT 116 cells, MOLT-4 cells, Jurkat cells, K-562 cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:350-1:1400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66556-1-Ig targets BCL10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12162

Product name: Recombinant human BCL10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-233 aa of BC053617

Sequence: MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ

相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 10
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
RRIDAB_2881917
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for BCL10 antibody 66556-1-IgDownload protocol
IF protocol for BCL10 antibody 66556-1-IgDownload protocol
IHC protocol for BCL10 antibody 66556-1-IgDownload protocol
WB protocol for BCL10 antibody 66556-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

High-expression of BCL10 inhibits cell-mediated immunity within the tumor immune microenvironment.

Authors - Jinyi Gu
View Article
humanWB

Biochem Biophys Res Commun

ZNF436 promotes tumor cell proliferation through transcriptional activation of BCL10 in glioma.

Authors - Yinwu Shang
View Article
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