Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
66178-1-Ig targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5661 Product name: Recombinant human AZGP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-298 aa of BC033830 Sequence: MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS 相同性解析による交差性が予測される生物種 |
| Full Name | alpha-2-glycoprotein 1, zinc-binding |
| Calculated molecular weight | 298 aa, 34 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC033830 |
| Gene Symbol | Zinc Alpha 2 Glycoprotein |
| Gene ID (NCBI) | 563 |
| RRID | AB_2881573 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P25311 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-Ig | Download protocol |
| IHC protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-Ig | Download protocol |
| WB protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Loss of EHMT2 enhances NK cell-driven anti-tumor immunity through TGF-β1 suppression.
| ||
Onco Targets Ther Reduction of AZGP1 predicts poor prognosis in esophageal squamous cell carcinoma patients in Northern China. |