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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human ovary cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human colorectal cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colorectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colon cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colon using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human rectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colon cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colorectal cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colorectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colorectal cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colorectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human ovary cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for IHC, ELISA
Cat No. 66172-2-Ig
Clone No.5B5A7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Synonyms

5B5A7, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC-Autostainer detected inhuman tonsillitis tissue, human stomach cancer tissue, human colorectal cancer tissue, human ovary cancer tissue, human appendicitis tissue
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human colorectal cancer tissue, human colon cancer tissue, human ovary cancer tissue, human stomach cancer tissue, human rectal cancer tissue, human tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66172-2-Ig targets MSH6 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12645

Product name: Recombinant human MSH6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC004246

Sequence: MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPE

相同性解析による交差性が予測される生物種
Full Name mutS homolog 6 (E. coli)
Calculated molecular weight 153 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC004246
Gene Symbol MSH6
Gene ID (NCBI) 2956
RRIDAB_3743303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP52701
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Protocols

Product Specific Protocols
IHC protocol for MSH6 antibody 66172-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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