Claudin 18 Monoclonal antibody

Claudin 18 Monoclonal Antibody for IP, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5G7F2

Cat no : 66167-1-Ig

Synonyms

claudin 18, CLDN18, SFTA5, SFTPJ



Tested Applications

Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman stomach tissue, human pancreas cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66167-1-Ig targets Claudin 18 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Claudin 18 fusion protein Ag15517
Full Name claudin 18
Calculated Molecular Weight 261 aa, 28 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC146668
Gene Symbol CLDN18
Gene ID (NCBI) 51208
RRIDAB_2881563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

Protocols

Product Specific Protocols
IHC protocol for Claudin 18 antibody 66167-1-IgDownload protocol
IP protocol for Claudin 18 antibody 66167-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Mucosal Immunol

Airway epithelium IgE-FcεRI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma

Authors - Chih-Ming Weng
IF

bioRxiv

Combined PI3K and MAPK inhibition synergizes to suppress PDAC

Authors - Bailey A Bye

World J Gastrointest Pathophysiol

Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry.

Authors - Nobuya Kurabe