Validation Data Gallery

Filter: AllWBIHCIF/ICCCytometric bead array sample testCytometric bead array

WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 66120-1-Ig (same clone as 66120-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51427-1
Cytometric bead array sample test of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS.
Cytometric bead array standard curve of MP51427-1
Cytometric bead array standard curve of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS. Standard:Ag6779. Range: 0.781-100 ng/mL.

Aldolase C Monoclonal antibody, PBS Only (Capture)

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66120-1-PBS

Clone No.5H7A5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66120-1-PBS targets Aldolase C as part of a matched antibody pair:

MP51427-1: 66120-1-PBS capture and 66120-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag6779 Product name: Recombinant human ALDOC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-364 aa of BC003613 Sequence: MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY 相同性解析による交差性が予測される生物種
Full Name	aldolase C, fructose-bisphosphate
Calculated molecular weight	39 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC003613
Gene Symbol	Aldolase C
Gene ID (NCBI)	230
RRID	AB_2881519
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P09972
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)

WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)

WB analysis of human brain using 66120-1-Ig (same clone as 66120-1-PBS)

IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)