Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66083-1-Ig targets TLE3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17906 Product name: Recombinant human TLE3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC015729 Sequence: MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI 相同性解析による交差性が予測される生物種 |
| Full Name | transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) |
| Calculated molecular weight | 772 aa, 83 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC015729 |
| Gene Symbol | TLE3 |
| Gene ID (NCBI) | 7090 |
| RRID | AB_11182267 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q04726 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TLE3 antibody 66083-1-Ig | Download protocol |
| IP protocol for TLE3 antibody 66083-1-Ig | Download protocol |
| WB protocol for TLE3 antibody 66083-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
