Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, fetal human brain tissue, SH-SY5Y cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
66076-1-Ig targets TTL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4708 Product name: Recombinant human TTL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-377 aa of BC036819 Sequence: MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKL 相同性解析による交差性が予測される生物種 |
| Full Name | tubulin tyrosine ligase |
| Calculated molecular weight | 377 aa, 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC036819 |
| Gene Symbol | TTL |
| Gene ID (NCBI) | 150465 |
| RRID | AB_11182924 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NG68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TTL antibody 66076-1-Ig | Download protocol |
| IHC protocol for TTL antibody 66076-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carcinogenesis Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing. | ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
| ||
J Cell Sci Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination | ||
Nat Commun α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments
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