Validation Data Gallery

Filter: AllWBIHC-AutostainerIHCIF-PIF/ICC

WB analysis using 66065-2-Ig
Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
WB analysis of human peripheral blood platelets using 66065-2-Ig
human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 66065-2-Ig
Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 66065-2-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 66065-2-Ig
THP-1 cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human appendicitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human pancreas using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human liver using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human pancreas cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human liver cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human stomach cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human oesophagus cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human placenta using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human liver cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66065-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human liver cancer using 66065-2-Ig
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human liver cancer using 66065-2-Ig
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human placenta using 66065-2-Ig
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 66065-2-Ig
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HUVEC using 66065-2-Ig
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).
IF Staining of HUVEC using 66065-2-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red).

CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66065-2-Ig

Clone No.3F8E2

Publications(137)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

3F8E2, EndoCAM, GPIIA, GPIIA', PECA1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (137)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human peripheral blood platelets, Jurkat cells, THP-1 cells
Positive IHC detected in	human liver cancer tissue, human oesophagus cancer tissue, human liver tissue, human tonsillitis tissue, human pancreas tissue, human placenta tissue, human pancreas cancer tissue, human colon cancer tissue, human liver cancer tissue, human appendicitis tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue, human liver cancer tissue
Positive IF/ICC detected in	HUVEC cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:5000-1:20000
Immunofluorescence (IF)-P	IF-P : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 17 publications below
IHC	See 29 publications below
IF	See 100 publications below

Product Information

66065-2-Ig targets CD31 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag19730 Product name: Recombinant human CD31 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 428-738 aa of BC022512 Sequence: EVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT 相同性解析による交差性が予測される生物種
Full Name	platelet/endothelial cell adhesion molecule
Calculated molecular weight	83 kDa
Observed molecular weight	120-130 kDa
GenBank accession number	BC022512
Gene Symbol	CD31
Gene ID (NCBI)	5175
ENSEMBL Gene ID	ENSG00000261371
RRID	AB_2918476
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P16284
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CD31 antibody 66065-2-Ig	Download protocol
IHC protocol for CD31 antibody 66065-2-Ig	Download protocol
WB protocol for CD31 antibody 66065-2-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cancer Cell Conserved spatial subtypes and cellular neighborhoods of cancer-associated fibroblasts revealed by single-cell spatial multi-omics Authors - Yunhe Liu View Article
human	WB	Cell ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization Authors - Yuqi Wang View Article
human	IF	Nat Nanotechnol Prevention of acute thrombosis with vascular endothelium antioxidative nanoscavenger. Authors - Yixin Zhong View Article
	IF	Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. Authors - Yizhi Zhan View Article
	IF	Bioact Mater Throw out an oligopeptide to catch a protein: Deep learning and natural language processing-screened tripeptide PSP promotes Osteolectin-mediated vascularized bone regeneration Authors - Yu Chen View Article
human	IF	Nat Commun Lactate bridges mesangial cells to the differentiation of follicular helper T cells in lupus nephritis. Authors - Mengdi Liu View Article

Validation Data Gallery

WB analysis using 66065-2-Ig

WB analysis of human peripheral blood platelets using 66065-2-Ig

WB analysis using 66065-2-Ig

WB analysis of Jurkat using 66065-2-Ig

WB analysis of THP-1 using 66065-2-Ig

IHC staining of human appendicitis using 66065-2-Ig

IHC staining of human pancreas using 66065-2-Ig

IHC staining of human liver using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human pancreas cancer using 66065-2-Ig

IHC staining of human liver cancer using 66065-2-Ig

IHC staining of human stomach cancer using 66065-2-Ig

IHC staining of human oesophagus cancer using 66065-2-Ig

IHC staining of human placenta using 66065-2-Ig

IHC staining of human liver cancer using 66065-2-Ig

IHC staining of human liver cancer using 66065-2-Ig

IHC staining of human breast cancer using 66065-2-Ig

IHC staining of human colon cancer using 66065-2-Ig

IHC staining of human liver using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human appendicitis using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human placenta using 66065-2-Ig

IHC staining of human oesophagus cancer using 66065-2-Ig

IHC staining of human liver cancer using 66065-2-Ig

IHC staining of human pancreas cancer using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human liver using 66065-2-Ig

IHC staining of human pancreas using 66065-2-Ig

IHC staining of human breast cancer using 66065-2-Ig

IHC staining of human breast cancer using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IHC staining of human tonsillitis using 66065-2-Ig

IF Staining of human liver cancer using 66065-2-Ig

IF Staining of human liver cancer using 66065-2-Ig

IF Staining of human placenta using 66065-2-Ig

IF Staining of human placenta using 66065-2-Ig

IF Staining of HUVEC using 66065-2-Ig

IF Staining of HUVEC using 66065-2-Ig

CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Conserved spatial subtypes and cellular neighborhoods of cancer-associated fibroblasts revealed by single-cell spatial multi-omics

ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization

Prevention of acute thrombosis with vascular endothelium antioxidative nanoscavenger.

Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis.

Throw out an oligopeptide to catch a protein: Deep learning and natural language processing-screened tripeptide PSP promotes Osteolectin-mediated vascularized bone regeneration

Lactate bridges mesangial cells to the differentiation of follicular helper T cells in lupus nephritis.

Able™

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