VIM Monoclonal antibody

VIM Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 65039-1-Ig

Synonyms

VIM



Tested Applications

Positive WB detected inA549 cells, A549
Positive IHC detected inLung cancer tissue, human lung cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65039-1-Ig targets VIM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name vimentin
Calculated Molecular Weight 54 kDa
GenBank Accession NumberBC066956
Gene Symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification MethodOther
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.