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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSH2 Monoclonal antibody (60161-2-Ig)

    WB analysis using 60161-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-2-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Saos-2 cells using MSH2 Monoclonal antibody (60161-2-Ig)

    WB analysis of Saos-2 using 60161-2-Ig

    Saos-2 cells were subjected to SDS PAGE followed by western blot with 60161-2-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MSH2 Monoclonal antibody (60161-2-Ig)

    WB analysis using 60161-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-2-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using MSH2 Monoclonal antibody (60161-2-Ig)

    IHC staining of human rectal cancer using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MSH2 Monoclonal antibody (60161-2-Ig)

    IHC staining of human appendicitis using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Ramos cells using MSH2 Monoclonal antibody (60161-2-Ig)

    IHC staining of Ramos using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH2 Monoclonal antibody (60161-2-Ig)

    IHC staining of human tonsillitis using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MSH2 Monoclonal antibody

MSH2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60161-2-Ig
Clone No.2E4A7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

2E4A7, MSH 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MDA-MB-468 cells, Saos-2 cells, COLO 320 cells, A431 cells, HuT 78 cells, MOLT-4 cells, K-562 cells
Positive IHC detected inRamos cells, human appendicitis tissue, human rectal cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60161-2-Ig targets MSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7835

Product name: Recombinant human MSH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 585-934 aa of BC021566

Sequence: SSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAILEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTALANQIPTVNNLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKALELEEFQYIGESQGYDIMEPAAKKCYLEREQGEKIIQEFLSKVKQMPFTEMSEENITIKLKQLKAEVIAKNNSFVNEIISRIKVTT

相同性解析による交差性が予測される生物種
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight105 kDa
GenBank accession numberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP43246
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
WB protocol for MSH2 antibody 60161-2-IgDownload protocol
IHC protocol for MSH2 antibody 60161-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols