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NR2F6 Monoclonal antibody

NR2F6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H2D11

Cat no : 60117-1-Ig

Synonyms

EAR 2, EAR2, ERBAL2, NR2F6, V erbA related protein 2



Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60117-1-Ig targets NR2F6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR2F6 fusion protein Ag7614
Full Name nuclear receptor subfamily 2, group F, member 6
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC002669
Gene Symbol NR2F6
Gene ID (NCBI) 2063
RRIDAB_2155654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development [PMID:15875024]. NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) [12690040]. It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity [PMID:18701084].

Protocols

Product Specific Protocols
WB protocol for NR2F6 antibody 60117-1-IgDownload protocol
IHC protocol for NR2F6 antibody 60117-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Sci Adv

Melanoma-intrinsic NR2F6 activity regulates antitumor immunity

Authors - Hyungsoo Kim
humanWB,IHC

Int J Mol Sci

NR2F6 Expression Correlates with Pelvic Lymph Node Metastasis and Poor Prognosis in Early-Stage Cervical Cancer.

Authors - Chunhao Niu
humanIHC

Int J Cancer

PMID: 30895619

Authors - Han Li
  • KD Validated