Serpina3k Polyclonal antibody

Serpina3k Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55480-1-AP

Synonyms

1300001I07Rik, contrapsin, D12Rp54, MMCM2, MMSpi2, RP54, Serpina3k, Spi 2, Spi2



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55480-1-AP targets Serpina3k in WB, IF, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name serine (or cysteine) peptidase inhibitor, clade A, member 3K
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberNM_011458
Gene Symbol Serpina3k
Gene ID (NCBI) 20714
RRIDAB_2881346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Serpina3k antibody 55480-1-APDownload protocol
IHC protocol for Serpina3k antibody 55480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Biol Toxicol

Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis.

Authors - Yaochen Cao
mouseWB

Int J Mol Sci

Sirtuin 7 Deficiency Reduces Inflammation and Tubular Damage Induced by an Episode of Acute Kidney Injury.

Authors - Andrea Sánchez-Navarro
ratWB

FASEB J

Transient response of serpinA3 during cellular stress.

Authors - Andrea Sánchez-Navarro
humanWB,IHC

Am J Physiol Renal Physiol

Urinary serpin-A3 is an early predictor of clinical response to therapy in patients with proliferative lupus nephritis

Authors - Miguel Ángel Martínez-Rojas
WB,IHC

Sci Rep

SerpinA3 in the Early Recognition of Acute Kidney Injury to Chronic Kidney Disease (CKD) transition in the rat and its Potentiality in the Recognition of Patients with CKD.

Authors - Andrea Sánchez-Navarro
mouseWB

Front Neurol

Neuroprotective Effects of Serpina3k in Traumatic Brain Injury.

Authors - Yao Jing