Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 55011-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 cells using 55011-1-AP
HAP1 (WT and HTRA1 KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
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HAP1 (WT and HTRA1 KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of mouse brain using 55011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HAP1 cells using 55011-1-AP
HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
×
HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
IHC staining of human placenta using 55011-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 55011-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human placenta using 55011-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 55011-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HTRA1 antibody (55011-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

HTRA1 Polyclonal antibody

HTRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55011-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.21.-, HtrA, PRSS11

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HAP1 cells, rat brain tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 10 publications below
IF	See 5 publications below

Product Information

55011-1-AP targets HTRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	HtrA serine peptidase 1
Calculated molecular weight	51 kDa
Observed molecular weight	48-50 kDa
GenBank accession number	NM_002775
Gene Symbol	HTRA1
Gene ID (NCBI)	5654
RRID	AB_10859830
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92743
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

Protocols

Product Specific Protocols
IF protocol for HTRA1 antibody 55011-1-AP	Download protocol
IHC protocol for HTRA1 antibody 55011-1-AP	Download protocol
WB protocol for HTRA1 antibody 55011-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cell Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures Authors - Yingjie Ding View Article
human, mouse	WB	J Clin Invest N-acetyl-l-leucine lowers α-synuclein levels and improves synaptic function in Parkinson's disease models. Authors - Pingping Song View Article
mouse		Biomaterials Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm Authors - Craig Jun Kit Wong View Article
human	WB	Cancer Biol Med VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy. Authors - Yanna Bi View Article
mouse,human	WB,IF	Cell Commun Signal Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration Authors - Wenchang Xu View Article
human	IHC,IF	Int J Mol Med Human papillomavirus E7 inhibits immune responses in keratinocytes by activating HTRA1‑mediated mitophagy. Authors - Boya Zhang View Article

Validation Data Gallery

WB analysis using 55011-1-AP

WB analysis of HAP1 cells using 55011-1-AP

WB analysis of mouse brain using 55011-1-AP

WB analysis of mouse brain using 55011-1-AP

IP experiment of HAP1 cells using 55011-1-AP

IHC staining of human placenta using 55011-1-AP

IHC staining of human placenta using 55011-1-AP

IHC staining of human placenta using 55011-1-AP

IF Staining of HepG2 using 55011-1-AP

HTRA1 Polyclonal antibody

HTRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures

N-acetyl-l-leucine lowers α-synuclein levels and improves synaptic function in Parkinson's disease models.

Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm

VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy.

Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration

Human papillomavirus E7 inhibits immune responses in keratinocytes by activating HTRA1‑mediated mitophagy.

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