Validation Data Gallery
Tested Applications
| Positive WB detected in | unboiled mouse brain tissue, unboiled mouse cerebellum tissue, unboiled rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31068-1-AP targets NTT4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34522 Product name: Recombinant human SLC6A17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 356-440 aa of NM_001010898 Sequence: GFKANIMNEKCVVENAEKILGYLNTNVLSRDLIPPHVNFSHLTTKDYMEMYNVIMTVKEDQFSALGLDPCLLEDELDKSVQGTGL 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 6, member 17 |
| Calculated molecular weight | 727 aa, 81 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | NM_001010898 |
| Gene Symbol | NTT4 |
| Gene ID (NCBI) | 388662 |
| RRID | AB_3669841 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H1V8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC6A17 also known as NTT4, is a member of the SLC6 family of transporters, which are responsible for the presynaptic uptake of most neurotransmitters. SLC6A17 is a transporter for neutral amino acids and is exclusively expressed in the brain(PMID: 23672601). Defects in SLC6A17 cause Intellectual developmental disorder, autosomal recessive 48(PMID: 25704603). For optimal WB detection with 31068-1-AP, we do not recommend boiling the sample after lysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NTT4 antibody 31068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |