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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK24 Polyclonal antibody (29846-1-AP)

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STK24 Polyclonal antibody (29846-1-AP)

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STK24 Polyclonal antibody (29846-1-AP)

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STK24 Polyclonal antibody (29846-1-AP)

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using STK24 Polyclonal antibody (29846-1-AP)

    IHC staining of mouse colon using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using STK24 Polyclonal antibody (29846-1-AP)

    IHC staining of human placenta using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STK24 Polyclonal antibody

STK24 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29846-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.11.1, Mammalian STE20-like protein kinase 3, MST3, Serine/threonine-protein kinase 24 12 kDa subunit, Serine/threonine-protein kinase 24 36 kDa subunit

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, Jurkat cells, rat lung tissue, rat skin tissue, rat spleen tissue
Positive IHC detected inmouse testis tissue, mouse colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29846-1-AP targets STK24 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31519

Product name: Recombinant human STK24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 320-443 aa of NM_003576

Sequence: SDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH

相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 24 (STE20 homolog, yeast)
Calculated molecular weight49KD
Observed molecular weight50 kDa
GenBank accession numberNM_003576
Gene Symbol STK24
Gene ID (NCBI) 8428
RRIDAB_2923614
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6E0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

Protocols

Product Specific Protocols
IHC protocol for STK24 antibody 29846-1-APDownload protocol
WB protocol for STK24 antibody 29846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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