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FTSJD2 Polyclonal antibody

FTSJD2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27707-1-AP

Synonyms

CMTR1, FTSJD2, hMTr1, ISG95, KIAA0082, MTr1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTSJD2 Polyclonal antibody (27707-1-AP)

    WB analysis using 27707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27707-1-AP (FTSJD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using FTSJD2 Polyclonal antibody (27707-1-AP)

    IHC staining of human testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using FTSJD2 Polyclonal antibody (27707-1-AP)

    IHC staining of human testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FTSJD2 Polyclonal antibody (27707-1-AP)

    IHC staining of mouse testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FTSJD2 Polyclonal antibody (27707-1-AP)

    IHC staining of mouse testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FTSJD2 Polyclonal antibody (27707-1-AP)

    IF Staining of U2OS using 27707-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 27707-1-AP (FTSJD2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inU-251 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27707-1-AP targets FTSJD2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTSJD2 fusion protein Ag26722
Full Name FtsJ methyltransferase domain containing 2
Calculated Molecular Weight 835 aa, 95 kDa
Observed Molecular Weight 96-100 kDa
GenBank Accession NumberBC031890
Gene Symbol FTSJD2
Gene ID (NCBI) 23070
RRIDAB_2880949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cap1 2′-O-methylation is catalyzed by cap methyltransferase 1 (CMTR1). CMTR1, also called IFN-stimulated gene 95 kDa protein (ISG95) or FTSJD2, is elevated by a viral infection and required to promote IFN-mediated induction of ISGs and the antiviral response. High CMTR1 expression is associated with poor prognosis in patients with CRC (PMID: 37024465). CMTr1 binds ATP-dependent RNA DHX15 helicase and that this interaction, mediated by the G-patch domain of CMTr1, has an advantageous effect on CMTr1 activity towards highly structured RNA substrates (PMID: 30397098).). Human CMTR1 is a 95 kDa nuclear protein that has multiple domains, consisting of a G-patch domain, a RrmJ/FtsJ methyltransferase domain, a non-functional cap guanylyltransferase-like domain and a WW domain (PMID: 30312682).

Protocols

Product Specific Protocols
WB protocol for FTSJD2 antibody 27707-1-APDownload protocol
IHC protocol for FTSJD2 antibody 27707-1-APDownload protocol
IF protocol for FTSJD2 antibody 27707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols