VEGFD Polyclonal antibody

VEGFD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26915-1-AP

Synonyms

c Fos induced growth factor, FIGF, VEGF D, VEGFD



Tested Applications

Positive WB detected inmouse heart tissue, MCF-7 cells, rat heart tissue
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human thyroid cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26915-1-AP is VEGFD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFD fusion protein Ag25552
Full Name c-fos induced growth factor (vascular endothelial growth factor D)
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027948
Gene Symbol FIGF
Gene ID (NCBI) 2277
RRIDAB_2880683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFD is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.

Protocols

Product Specific Protocols
WB protocol for VEGFD antibody 26915-1-APDownload protocol
IHC protocol for VEGFD antibody 26915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Gastric Cancer

Cathepsin L promotes angiogenesis by regulating the CDP/Cux/VEGF-D pathway in human gastric cancer.

Authors - Tao Pan
mouseIHC

Cereb Cortex

Prolonged Consumption of Sweetened Beverages Lastingly Deteriorates Cognitive Functions and Reward Processing in Mice.

Authors - Héloïse Hamelin
ratIF

FASEB J

VEGF-D-mediated signaling in tendon cells is involved in degenerative processes.

Authors - Herbert Tempfer
mouseIHC

Ann Transl Med

Clostridium butyricum alleviates dextran sulfate sodium-induced experimental colitis and promotes intestinal lymphatic vessel regeneration in mice.

Authors - Xing Chen
mouseIHC

BMC Complement Med Ther

Effects of plant-based medicinal food on postoperative recurrence and lung metastasis of gastric cancer regulated by Wnt/β-catenin-EMT signaling pathway and VEGF-C/D-VEGFR-3 cascade in a mouse model

Authors - Lin Tian
mouse,humanWB,IF

Biochim Biophys Acta Mol Basis Dis

WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization

Authors - Yanhui Bai