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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TP53RK Polyclonal antibody (26818-1-AP)

    WB analysis of Jurkat using 26818-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26818-1-AP (TP53RK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TP53RK Polyclonal antibody (26818-1-AP)

    WB analysis of HepG2 using 26818-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26818-1-AP (TP53RK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TP53RK Polyclonal antibody (26818-1-AP)

    IP experiment of Jurkat using 26818-1-AP

    IP result of anti-TP53RK (IP:26818-1-AP, 4ug; Detection:26818-1-AP 1:700) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of human testis using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of human testis using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of human placenta using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of human stomach cancer using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of mouse heart using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of mouse stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of rat heart using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of rat stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of mouse stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of rat stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

TP53RK Polyclonal antibody

TP53RK Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26818-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

Atypical serine/threonine protein kinase TP53RK, BUD32, C20orf64, EC:2.7.11.1, EC:3.6.-.-

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue, rat stomach tissue, human stomach cancer tissue, mouse heart tissue, rat heart tissue, mouse stomach tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26818-1-AP targets TP53RK in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25326

Product name: Recombinant human TP53RK protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 154-253 aa of BC009727

Sequence: HDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG

相同性解析による交差性が予測される生物種
Full Name TP53 regulating kinase
Calculated molecular weight 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009727
Gene Symbol TP53RK
Gene ID (NCBI) 112858
RRIDAB_2880647
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TP53RK antibody 26818-1-APDownload protocol
IP protocol for TP53RK antibody 26818-1-APDownload protocol
WB protocol for TP53RK antibody 26818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Med

Systematic screening for potential therapeutic targets in osteosarcoma through a kinome-wide CRISPR-Cas9 library.

Authors - Yuanzhong Wu
  • KO Validated
View Article

Cell Rep

Integrative 3D genome and epigenomic analysis identifies TP53RK and SYBU as critical regulators of stemness and anti-tumor immunity in HCC.

Authors - Yanmei Yang
View Article
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