HKDC1 Polyclonal antibody

HKDC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25874-1-AP

Synonyms

hexokinase domain containing 1, HKDC1, Putative hexokinase HKDC1



Tested Applications

Positive WB detected inA549 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25874-1-AP is HKDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HKDC1 fusion protein Ag23045
Full Name hexokinase domain containing 1
Calculated Molecular Weight 917 aa, 103 kDa
Observed Molecular Weight 102 kDa, 82-90 kDa
GenBank Accession NumberBC110504
Gene Symbol HKDC1
Gene ID (NCBI) 80201
RRIDAB_2880279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HKDC1 antibody 25874-1-APDownload protocol
IHC protocol for HKDC1 antibody 25874-1-APDownload protocol
IP protocol for HKDC1 antibody 25874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virol Sin

Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.

Authors - Yingying Song
humanIHC,IF

Int J Mol Sci

Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer

Authors - Jia Dong
mouseWB,IHC

J Mol Histol

New insights into the treatment of polycystic ovary syndrome: HKDC1 promotes the growth of ovarian granulocyte cells by regulating mitochondrial function and glycolysis

Authors - Peiwei Cong
  • KD Validated
humanWB

J Cancer

HKDC1 enhances the proliferation, migration and glycolysis of pancreatic adenocarcinoma and is linked to immune infiltration

Authors - Qiang Pang