Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
25556-1-AP targets ZNF695 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22202 Product name: Recombinant human ZNF695 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-133 aa of BC023527 Sequence: LSSYLTEDILPEQGLQVSFQKVMLRRYERCCLEKLRLRNDWEIVAM 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 695 |
| Calculated molecular weight | 515 aa, 60 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC023527 |
| Gene Symbol | ZNF695 |
| Gene ID (NCBI) | 57116 |
| RRID | AB_2880130 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IW36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF695 is a KRAB-containing zinc finger transcription factor localized in the nucleus. It binds DNA and mediates transcriptional repression, and its dysregulation participates in tumor progression across multiple malignancies. Elevated ZNF695 promotes cancer cell proliferation, accelerates cell cycle and suppresses apoptosis by activating NEK2 and PI3K/Akt/mTOR pathways. It acts as a prognostic biomarker correlated with tumor immune infiltration in colorectal, cervical, breast and endometrial cancers. Its expression varies among tumor subtypes, associating with aggressive tumor phenotypes (PMID: 38441026; PMID: 40682845).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF695 antibody 25556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Rep Zinc finger protein 695 facilitates the proliferation of colorectal cancer cells through activation of the NEK2 and PI3K/Akt/mTOR signaling pathways
| ||
J Cancer Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma. |